The Cologne Center for Genomics has been acquired leading-edge technologies to help research groups of the University of Cologne finding answers in biology and medicine. The following table gives you a short overview about available platforms and their major application areas in regard to capillary electrophoresis and genotyping.

Comparison of SNP genotyping platforms available at the CCG

On the CCG many genotyping methods can be performed. These can be grouped according to their plex level.

A typical GWA study of thousands of samples could for example start with high plex arrays from Illumina and Affymetrix. Such studies typically provide a number (typically many hundreds) of positive signals that need to be verified in other collectives. Therefore other methods with a low/medium plex level must be used - eg SNPstream and GoldenGate. TaqMan OpenArray as a single plex method uses a low volume approach to step into the field that low plex methods normally fill out.

Finally, as a rule, some important or difficult SNPs remain that are genotyped with single plex procedures - such as TaqMan/OpenArray or pyrosequencing.

This strategy maximizes the amount of genetic information that can be achieved with the available study resources.

* Current human high end array as en example; other products are available
** throughput on the CCG platforms

Contact

email: ccg-service@uni-koeln.de